PDBe 1ku7

X-ray diffraction
2.4Å resolution

Crystal Structure of Thermus aquatics RNA Polymerase SigmaA Subunit Region 4 Bound to-35 Element DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
RNA polymerase sigma factor SigA Chains: A, D
Molecule details ›
Chains: A, D
Length: 73 amino acids
Theoretical weight: 8.74 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EZJ8 (Residues: 366-438; Coverage: 17%)
Gene name: sigA
Sequence domains: Sigma-70, region 4
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*CP*CP*TP*TP*GP*AP*CP*AP*AP*AP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 11 nucleotides
Theoretical weight: 3.34 KDa
Source organism: Thermus aquaticus
Expression system: Not provided
5'-D(*CP*CP*TP*TP*TP*GP*TP*CP*AP*AP*G)-3' Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.32 KDa
Source organism: Thermus aquaticus
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9A
Spacegroup: P212121
Unit cell:
a: 36.18Å b: 62.65Å c: 94.81Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.255 0.293
Expression systems:
  • Escherichia coli
  • Not provided