PDBe 1ksy

X-ray diffraction
3.05Å resolution

Crystal Structures of Two Intermediates in the Assembly of the Papillomavirus Replication Initiation Complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Replication protein E1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 154 amino acids
Theoretical weight: 17.57 KDa
Source organism: Bovine papillomavirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Best match: P03116 (Residues: 159-309)
Gene name: E1
E1 Recognition Sequence, Strand 1 Chains: D, E
Molecule details ›
Chains: D, E
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Bovine papillomavirus
Expression system: Not provided
E1 Recognition Sequence, Strand 2 Chain: F
Molecule details ›
Chain: F
Length: 21 nucleotides
Theoretical weight: 6.44 KDa
Source organism: Bovine papillomavirus
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X26C
Spacegroup: C2
Unit cell:
a: 149.111Å b: 110.6Å c: 75.084Å
α: 90° β: 116.85° γ: 90°
R-values:
R R work R free
0.277 0.277 0.316
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided