› 1jxg

X-ray diffraction
1.6Å resolution

The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge

Released:
DOI: 10.2210/pdb1jxg/pdb
PDB entry 1jxg coloured by chain, front view.
PDB entry 1jxg coloured by chain, side view.
PDB entry 1jxg coloured by chain, top view.
Source organism: Populus nigra
Primary publication:
The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge.
Milani M, Andolfi L, Cannistraro S, Verbeet MP, Bolognesi M
Acta Crystallogr D Biol Crystallogr 57 1735-8 (2001)
PMID: 11679761

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132379 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Plastocyanin A, chloroplastic Chains: A, B
Molecule details ›
Chains: A, B
Length: 100 amino acids
Theoretical weight: 10.59 KDa
Source organism: Populus nigra
Expression system: Escherichia coli
UniProt:
  • Canonical: P00299 (Residues: 70-168; Coverage: 59%)
Gene name: PETE
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

2 bound ligands:
Ligand CU1 2 x CU1
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 47.381Å b: 49.306Å c: 76.576Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.148 0.145 0.205
Expression system: Escherichia coli

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Citations

6 citation in other articles

Multi-template approach to modeling engineered disulfide bonds.
Pellequer et al. (2006)
5 more