PDBe 1jva

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE VMA1-DERIVED ENDONUCLEASE BEARING THE N AND C EXTEIN PROPEPTIDES

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O + H(+)(In) = ADP + phosphate + H(+)(Out). 
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease PI-SceI Chains: A, B
Molecule details ›
Chains: A, B
Length: 475 amino acids
Theoretical weight: 53.15 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17255 (Residues: 273-747; Coverage: 44%)
Gene names: CLS8, D1286, TFP1, VMA1, YDL185W
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P1
Unit cell:
a: 66.178Å b: 68.846Å c: 58.524Å
α: 103.1° β: 98.79° γ: 78.98°
R-values:
R R work R free
0.2 0.198 0.24
Expression system: Escherichia coli BL21(DE3)