Solution NMR

NMR Structure of P/CAF Bromodomain in Complex with HIV-1 Tat Peptide


Function and Biology Details

Reactions catalysed:
Acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N(6)-acetyl-L-lysine
Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein Tat Chain: A
Molecule details ›
Chain: A
Length: 11 amino acids
Theoretical weight: 1.48 KDa
Source organism: Human immunodeficiency virus type 1 (BRU ISOLATE)
Expression system: Not provided
  • Canonical: P04610 (Residues: 46-55; Coverage: 12%)
Gene name: tat
Histone acetyltransferase KAT2B Chain: B
Molecule details ›
Chain: B
Length: 118 amino acids
Theoretical weight: 14.05 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q92831 (Residues: 719-832; Coverage: 14%)
Gene names: KAT2B, PCAF
Sequence domains: Bromodomain
Structure domains: Bromodomain-like

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Refinement method: Distance Geometry/Simulated Annealing
Chemical shifts: BMR4312  
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)