› 1jjt

X-ray diffraction
1.8Å resolution

IMP-1 METALLO BETA-LACTAMASE FROM PSEUDOMONAS AERUGINOSA IN COMPLEX WITH A BIARYL SUCCINIC ACID INHIBITOR (1)

Released:
DOI: 10.2210/pdb1jjt/pdb
PDB entry 1jjt coloured by chain, front view.
PDB entry 1jjt coloured by chain, side view.
PDB entry 1jjt coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Succinic acids as potent inhibitors of plasmid-borne IMP-1 metallo-beta-lactamase.
Toney JH, Hammond GG, Fitzgerald PM, Sharma N, Balkovec JM, Rouen GP, Olson SH, Hammond ML, Greenlee ML, Gao YD
J Biol Chem 276 31913-8 (2001)
PMID: 11390410

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-181271 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.15 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q79MP6 (Residues: 19-246; Coverage: 100%)
Gene names: bla IMP, bla-imp, blaESP, blaIMP-1, imp
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

3 bound ligands:
Ligand ZN 6 x ZN
Ligand ACT 2 x ACT
Ligand BDS 2 x BDS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P21
Unit cell:
a: 71.8Å b: 45.18Å c: 64.51Å
α: 90° β: 100.32° γ: 90°
R-values:
R R work R free
0.199 not available 0.296
Expression system: Escherichia coli

Quick links

Citations

14 review citations

Metallo-beta-lactamases: the quiet before the storm?
Walsh et al. (2005)
13 more

12 mentions without citation

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
11 more