1jfi

X-ray diffraction
2.62Å resolution

Crystal Structure of the NC2-TBP-DNA Ternary Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
Dr1-associated corepressor Chain: A
Molecule details ›
Chain: A
Length: 98 amino acids
Theoretical weight: 10.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q14919 (Residues: 1-77; Coverage: 38%)
Gene name: DRAP1
Sequence domains: Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Structure domains: Histone, subunit A
Protein Dr1 Chain: B
Molecule details ›
Chain: B
Length: 179 amino acids
Theoretical weight: 19.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q01658 (Residues: 1-176; Coverage: 100%)
Gene name: DR1
Sequence domains: Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Structure domains: Histone, subunit A
TATA-box-binding protein Chain: C
Molecule details ›
Chain: C
Length: 185 amino acids
Theoretical weight: 20.86 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20226 (Residues: 159-339; Coverage: 53%)
Gene names: GTF2D1, TBP, TF2D, TFIID
Sequence domains: Transcription factor TFIID (or TATA-binding protein, TBP)
Structure domains: TATA-Binding Protein
5'-D(*TP*TP*GP*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*GP*CP*TP*CP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 5.84 KDa
5'-D(*G*GP*AP*GP*CP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*CP*CP*AP*A)-3' Chain: E
Molecule details ›
Chain: E
Length: 19 nucleotides
Theoretical weight: 5.8 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2221
Unit cell:
a: 76.684Å b: 119.075Å c: 155.076Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.239 0.233 0.277
Expression system: Escherichia coli BL21(DE3)