1jd6

X-ray diffraction
2.7Å resolution

Crystal Structure of DIAP1-BIR2/Hid Complex

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Death-associated inhibitor of apoptosis 1 Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 14.08 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q24306 (Residues: 201-323; Coverage: 28%)
Gene names: CG12284, Diap1, Iap1, th
Sequence domains: Inhibitor of Apoptosis domain
Structure domains: Inhibitor Of Apoptosis Protein (2mihbC-IAP-1); Chain A
Cell death protein hid Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.05 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
UniProt:
  • Canonical: Q24106 (Residues: 2-11; Coverage: 2%)
Gene names: CG5123, W, hid

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P6522
Unit cell:
a: 62.7Å b: 62.7Å c: 130.7Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.22 0.217 0.287
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided