› 1j9c

X-ray diffraction
2.9Å resolution

Crystal Structure of tissue factor-factor VIIa complex

Released:
DOI: 10.2210/pdb1j9c/pdb
PDB entry 1j9c coloured by chain, front view.
PDB entry 1j9c coloured by chain, side view.
PDB entry 1j9c coloured by chain, top view.
Source organism: Homo sapiens
Entry authors: Huang M, Ruf W, Edgington TS, Wilson IA

Function and Biology Details

Reaction catalysed: 
Selective cleavage of Arg-|-Ile bond in factor X to form factor Xa.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140352 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tissue factor Chain: T
Molecule details ›
Chain: T
Length: 210 amino acids
Theoretical weight: 23.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13726 (Residues: 33-242; Coverage: 80%)
Gene name: F3
Sequence domains:
Structure domains: Immunoglobulins
Factor VII light chain Chain: L
Molecule details ›
Chain: L
Length: 95 amino acids
Theoretical weight: 10.37 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P08709 (Residues: 108-202; Coverage: 21%)
Gene name: F7
Sequence domains:
Structure domains: Laminin
Factor VII heavy chain Chain: H
Molecule details ›
Chain: H
Length: 254 amino acids
Theoretical weight: 28.1 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P08709 (Residues: 213-466; Coverage: 57%)
Gene name: F7
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

5 bound ligands:
Ligand GLC 1 x GLC
Ligand FUL 1 x FUL
Ligand 0Z6 1 x 0Z6
Ligand NAG 1 x NAG
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 77.038Å b: 68.302Å c: 78.438Å
α: 90° β: 92.12° γ: 90°
R-values:
R R work R free
0.221 0.221 0.299
Expression systems:
  • Escherichia coli
  • Cricetulus griseus

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