X-ray diffraction
1.8Å resolution

Structure of the N-terminal Exonuclease Domain of the Epsilon Subunit of E.coli DNA Polymerase III at pH 8.5


Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
DNA polymerase III subunit epsilon Chain: A
Molecule details ›
Chain: A
Length: 186 amino acids
Theoretical weight: 20.74 KDa
Source organism: Escherichia coli
Expression system: Enterobacteria phage T7
  • Canonical: P03007 (Residues: 1-186; Coverage: 77%)
Gene names: JW0205, b0215, dnaQ, mutD
Sequence domains: Exonuclease
Structure domains: Ribonuclease H-like superfamily/Ribonuclease H

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P41212
Unit cell:
a: 60.8Å b: 60.8Å c: 111.1Å
α: 90° β: 90° γ: 90°
R R work R free
0.199 0.199 0.227
Expression system: Enterobacteria phage T7