1j34

X-ray diffraction
1.55Å resolution

Crystal Structure of Mg(II)-and Ca(II)-bound Gla Domain of Factor IX Complexed with Binding Protein

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Snaclec coagulation factor IX-binding protein subunit A Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.66 KDa
Source organism: Protobothrops flavoviridis
UniProt:
  • Canonical: Q7LZ71 (Residues: 1-129; Coverage: 100%)
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A
Snaclec coagulation factor IX/factor X-binding protein subunit B Chain: B
Molecule details ›
Chain: B
Length: 123 amino acids
Theoretical weight: 14.46 KDa
Source organism: Protobothrops flavoviridis
UniProt:
  • Canonical: P23807 (Residues: 24-146; Coverage: 100%)
Sequence domains: Lectin C-type domain
Structure domains: Mannose-Binding Protein A, subunit A
Coagulation factor IXa light chain Chain: C
Molecule details ›
Chain: C
Length: 46 amino acids
Theoretical weight: 6.18 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00741 (Residues: 47-92; Coverage: 11%)
Gene name: F9
Sequence domains: Vitamin K-dependent carboxylation/gamma-carboxyglutamic (GLA) domain

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: C2
Unit cell:
a: 128.552Å b: 37.204Å c: 62.626Å
α: 90° β: 103.56° γ: 90°
R-values:
R R work R free
0.185 0.183 0.212