1ixs

X-ray diffraction
3.2Å resolution

Structure of RuvB complexed with RuvA domain III

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Holliday junction ATP-dependent DNA helicase RuvA Chain: A
Molecule details ›
Chain: A
Length: 62 amino acids
Theoretical weight: 6.7 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9F1Q3 (Residues: 130-191; Coverage: 33%)
Gene names: TTHA0291, ruvA
Sequence domains: RuvA, C-terminal domain
Structure domains: DNA helicase RuvA subunit, C-terminal domain
Holliday junction ATP-dependent DNA helicase RuvB Chain: B
Molecule details ›
Chain: B
Length: 318 amino acids
Theoretical weight: 35.42 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SL87 (Residues: 1-318; Coverage: 98%)
Gene names: TTHA0406, ruvB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL24XU
Spacegroup: P43212
Unit cell:
a: 102.06Å b: 102.06Å c: 137.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.231 0.231 0.294
Expression system: Escherichia coli BL21(DE3)