› 1it0

X-ray diffraction
2Å resolution

Crystal structure of xylanase from Streptomyces olivaceoviridis E-86 complexed with lactose

Released:
DOI: 10.2210/pdb1it0/pdb
PDB entry 1it0 coloured by chain, front view.
PDB entry 1it0 coloured by chain, side view.
PDB entry 1it0 coloured by chain, top view.
Source organism: Streptomyces olivaceoviridis
Primary publication:
Crystal structures of the sugar complexes of Streptomyces olivaceoviridis E-86 xylanase: sugar binding structure of the family 13 carbohydrate binding module.
Fujimoto Z, Kuno A, Kaneko S, Kobayashi H, Kusakabe I, Mizuno H
J Mol Biol 316 65-78 (2002)
PMID: 11829503

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Beta-xylanase Chains: A, B
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 46.79 KDa
Source organism: Streptomyces olivaceoviridis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7SI98 (Residues: 1-436; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC, GAL
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 79.54Å b: 95.25Å c: 141.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.191 0.224
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
4 more

1 mention without citation

TINAGL1 and B3GALNT1 are potential therapy target genes to suppress metastasis in non-small cell lung cancer.
Umeyama et al. (2014)