› 1iru

X-ray diffraction
2.75Å resolution

Crystal Structure of the mammalian 20S proteasome at 2.75 A resolution

Released:
DOI: 10.2210/pdb1iru/pdb
PDB entry 1iru coloured by chain, front view.
PDB entry 1iru coloured by chain, side view.
PDB entry 1iru coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The structure of the mammalian 20S proteasome at 2.75 A resolution.
Unno M, Mizushima T, Morimoto Y, Tomisugi Y, Tanaka K, Yasuoka N, Tsukihara T
Structure 10 609-18 (2002)
PMID: 12015144

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero 28-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152633 (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Proteasome subunit alpha type-6 Chains: A, O
Molecule details ›
Chains: A, O
Length: 246 amino acids
Theoretical weight: 27.43 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2YDE4 (Residues: 1-246; Coverage: 100%)
Gene name: PSMA6
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-2 Chains: B, P
Molecule details ›
Chains: B, P
Length: 233 amino acids
Theoretical weight: 25.8 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T0Y5 (Residues: 2-234; Coverage: 100%)
Gene name: PSMA2
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-4 Chains: C, Q
Molecule details ›
Chains: C, Q
Length: 261 amino acids
Theoretical weight: 29.53 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3ZCK9 (Residues: 1-261; Coverage: 100%)
Gene name: PSMA4
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-7 Chains: D, R
Molecule details ›
Chains: D, R
Length: 248 amino acids
Theoretical weight: 27.93 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3ZBG0 (Residues: 1-248; Coverage: 100%)
Gene name: PSMA7
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-5 Chains: E, S
Molecule details ›
Chains: E, S
Length: 241 amino acids
Theoretical weight: 26.49 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q5E987 (Residues: 1-241; Coverage: 100%)
Gene name: PSMA5
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-1 Chains: F, T
Molecule details ›
Chains: F, T
Length: 263 amino acids
Theoretical weight: 29.6 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T0X5 (Residues: 1-263; Coverage: 100%)
Gene name: PSMA1
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit alpha type-3 Chains: G, U
Molecule details ›
Chains: G, U
Length: 254 amino acids
Theoretical weight: 28.34 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q58DU5 (Residues: 2-255; Coverage: 100%)
Gene name: PSMA3
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-6 Chains: H, V
Molecule details ›
Chains: H, V
Length: 205 amino acids
Theoretical weight: 21.92 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3MHN0 (Residues: 35-238; Coverage: 85%)
Gene name: PSMB6
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-7 Chains: I, W
Molecule details ›
Chains: I, W
Length: 234 amino acids
Theoretical weight: 25.35 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2TBP0 (Residues: 44-277; Coverage: 85%)
Gene name: PSMB7
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-3 Chains: J, X
Molecule details ›
Chains: J, X
Length: 205 amino acids
Theoretical weight: 22.95 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P33672 (Residues: 1-205; Coverage: 100%)
Gene name: PSMB3
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-2 Chains: K, Y
Molecule details ›
Chains: K, Y
Length: 201 amino acids
Theoretical weight: 22.86 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q5E9K0 (Residues: 1-201; Coverage: 100%)
Gene name: PSMB2
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-5 Chains: L, Z
Molecule details ›
Chains: L, Z
Length: 204 amino acids
Theoretical weight: 22.48 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q32KL2 (Residues: 60-262; Coverage: 77%)
Gene name: PSMB5
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-1 Chains: 1, M
Molecule details ›
Chains: 1, M
Length: 213 amino acids
Theoretical weight: 23.58 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2TBX6 (Residues: 29-241; Coverage: 88%)
Gene name: PSMB1
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta type-4 Chains: 2, N
Molecule details ›
Chains: 2, N
Length: 219 amino acids
Theoretical weight: 24.4 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q3T108 (Residues: 46-264; Coverage: 83%)
Gene name: PSMB4
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1

Ligands and Environments

1 bound ligand:
Ligand MG 30 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 316.7Å b: 205.9Å c: 116Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.25 0.294

Quick links

Citations

90 review citations

Recognition and processing of ubiquitin-protein conjugates by the proteasome.
Finley D. (2009)
89 more

32 mentions without citation

Computational Approaches for the Discovery of Human Proteasome Inhibitors: An Overview.
Guedes et al. (2016)
31 more