› 1iqd

X-ray diffraction
2Å resolution

Human Factor VIII C2 Domain complexed to human monoclonal BO2C11 Fab.

Released:
DOI: 10.2210/pdb1iqd/pdb
PDB entry 1iqd coloured by chain, front view.
PDB entry 1iqd coloured by chain, side view.
PDB entry 1iqd coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of a factor VIII C2 domain-immunoglobulin G4kappa Fab complex: identification of an inhibitory antibody epitope on the surface of factor VIII.
Spiegel PC, Jacquemin M, Saint-Remy JM, Stoddard BL, Pratt KP
Blood 98 13-9 (2001)
PMID: 11418455

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-208207 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HUMAN MONOCLONAL BO2C11 FAB LIGHT CHAIN Chain: A
Molecule details ›
Chain: A
Length: 211 amino acids
Theoretical weight: 22.92 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
HUMAN MONOCLONAL BO2C11 FAB HEAVY CHAIN Chain: B
Molecule details ›
Chain: B
Length: 215 amino acids
Theoretical weight: 22.74 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
Factor VIIIa light chain Chain: C
Molecule details ›
Chain: C
Length: 156 amino acids
Theoretical weight: 17.74 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00451 (Residues: 2193-2348; Coverage: 7%)
Gene names: F8, F8C
Sequence domains: F5/8 type C domain
Structure domains: Galactose-binding domain-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 64.535Å b: 73.701Å c: 112.395Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.253
Expression system: Komagataella pastoris

Quick links

Citations

20 review citations

Inhibitors: resolving diagnostic and therapeutic dilemmas.
Dimichele D. (2002)
19 more

46 mentions without citation

Design and engineering of deimmunized biotherapeutics.
Griswold et al. (2016)
45 more