› 1igc

X-ray diffraction
2.6Å resolution

IGG1 FAB FRAGMENT (MOPC21) COMPLEX WITH DOMAIN III OF PROTEIN G FROM STREPTOCOCCUS

Released:
DOI: 10.2210/pdb1igc/pdb
PDB entry 1igc coloured by chain, front view.
PDB entry 1igc coloured by chain, side view.
PDB entry 1igc coloured by chain, top view.
Source organisms:
Primary publication:
The third IgG-binding domain from streptococcal protein G. An analysis by X-ray crystallography of the structure alone and in a complex with Fab.
Derrick JP, Wigley DB
J Mol Biol 243 906-18 (1994)
PMID: 7966308

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-208464 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig kappa chain V-V region MOPC 21 Chain: L
Molecule details ›
Chain: L
Length: 213 amino acids
Theoretical weight: 23.56 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01634 (Residues: 30-136; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
IGG1-KAPPA MOPC21 FAB (HEAVY CHAIN) Chain: H
Molecule details ›
Chain: H
Length: 222 amino acids
Theoretical weight: 23.86 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 61 amino acids
Theoretical weight: 6.66 KDa
Source organism: Streptococcus sp. G148
Expression system: Escherichia coli
UniProt:
  • Canonical: P06654 (Residues: 292-352; Coverage: 15%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 64.5Å b: 70.5Å c: 120.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.168 not available not available
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Weak alignment offers new NMR opportunities to study protein structure and dynamics.
Bax A. (2003)
10 more

31 mentions without citation

Broadly neutralizing antibody PGT121 allosterically modulates CD4 binding via recognition of the HIV-1 gp120 V3 base and multiple surrounding glycans.
Julien et al. (2013)
30 more