1id8

Solution NMR

NMR STRUCTURE OF GLUTAMATE MUTASE (B12-BINDING SUBUNIT) COMPLEXED WITH THE VITAMIN B12 NUCLEOTIDE

Released:

Function and Biology Details

Reaction catalysed:
L-threo-3-methylaspartate = L-glutamate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate mutase sigma subunit Chain: A
Molecule details ›
Chain: A
Length: 137 amino acids
Theoretical weight: 14.76 KDa
Source organism: Clostridium tetanomorphum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q05488 (Residues: 1-137; Coverage: 100%)
Gene names: glmS, mutS
Sequence domains: B12 binding domain
Structure domains: Cobalamin-binding domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: restrained simulated annealing, molecular dynamics, energy minimazation
Expression system: Escherichia coli BL21(DE3)