PDBe 1i94

X-ray diffraction
3.2Å resolution

CRYSTAL STRUCTURES OF THE SMALL RIBOSOMAL SUBUNIT WITH TETRACYCLINE, EDEINE AND IF3

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 21-mer (preferred)
Entry contents:
20 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (21 distinct):
30S RIBOSOMAL PROTEIN S2 Chain: B
Molecule details ›
Chain: B
Length: 255 amino acids
Theoretical weight: 29.19 KDa
Source organism: Thermus thermophilus
Structure domains:
30S RIBOSOMAL PROTEIN S3 Chain: C
Molecule details ›
Chain: C
Length: 238 amino acids
Theoretical weight: 26.62 KDa
Source organism: Thermus thermophilus
Structure domains:
30S RIBOSOMAL PROTEIN S4 Chain: D
Molecule details ›
Chain: D
Length: 208 amino acids
Theoretical weight: 24.24 KDa
Source organism: Thermus thermophilus
Structure domains:
30S RIBOSOMAL PROTEIN S5 Chain: E
Molecule details ›
Chain: E
Length: 161 amino acids
Theoretical weight: 17.45 KDa
Source organism: Thermus thermophilus
Structure domains:
30S RIBOSOMAL PROTEIN S6 Chain: F
Molecule details ›
Chain: F
Length: 101 amino acids
Theoretical weight: 11.99 KDa
Source organism: Thermus thermophilus
Structure domains: Alpha-Beta Plaits
30S RIBOSOMAL PROTEIN S7 Chain: G
Molecule details ›
Chain: G
Length: 155 amino acids
Theoretical weight: 17.92 KDa
Source organism: Thermus thermophilus
Structure domains: Ribosomal protein S7 domain
30S RIBOSOMAL PROTEIN S8 Chain: H
Molecule details ›
Chain: H
Length: 138 amino acids
Theoretical weight: 15.89 KDa
Source organism: Thermus thermophilus
Structure domains:
30S RIBOSOMAL PROTEIN S9 Chain: I
Molecule details ›
Chain: I
Length: 128 amino acids
Theoretical weight: 14.43 KDa
Source organism: Thermus thermophilus
Structure domains: Ribosomal Protein S5; domain 2
30S RIBOSOMAL PROTEIN S10 Chain: J
Molecule details ›
Chain: J
Length: 104 amino acids
Theoretical weight: 11.82 KDa
Source organism: Thermus thermophilus
Structure domains: Ribosomal protein S10 domain
30S RIBOSOMAL PROTEIN S11 Chain: K
Molecule details ›
Chain: K
Length: 128 amino acids
Theoretical weight: 13.61 KDa
Source organism: Thermus thermophilus
Structure domains: Ribosomal protein S11
30S RIBOSOMAL PROTEIN S12 Chain: L
Molecule details ›
Chain: L
Length: 131 amino acids
Theoretical weight: 14.51 KDa
Source organism: Thermus thermophilus
Structure domains: Nucleic acid-binding proteins
30S RIBOSOMAL PROTEIN S13 Chain: M
Molecule details ›
Chain: M
Length: 125 amino acids
Theoretical weight: 14.21 KDa
Source organism: Thermus thermophilus
Structure domains:
30S RIBOSOMAL PROTEIN S14 Chain: N
Molecule details ›
Chain: N
Length: 60 amino acids
Theoretical weight: 7.03 KDa
Source organism: Thermus thermophilus
Structure domains: 30s Ribosomal Protein S14; Chain N
30S RIBOSOMAL PROTEIN S15 Chain: O
Molecule details ›
Chain: O
Length: 88 amino acids
Theoretical weight: 10.45 KDa
Source organism: Thermus thermophilus
Structure domains: S15/NS1, RNA-binding
30S RIBOSOMAL PROTEIN S16 Chain: P
Molecule details ›
Chain: P
Length: 88 amino acids
Theoretical weight: 10.41 KDa
Source organism: Thermus thermophilus
Structure domains: S16 Ribosomal Protein; Chain: A;
30S RIBOSOMAL PROTEIN S17 Chain: Q
Molecule details ›
Chain: Q
Length: 104 amino acids
Theoretical weight: 12.14 KDa
Source organism: Thermus thermophilus
Structure domains: Nucleic acid-binding proteins
30S RIBOSOMAL PROTEIN S18 Chain: R
Molecule details ›
Chain: R
Length: 87 amino acids
Theoretical weight: 10.11 KDa
Source organism: Thermus thermophilus
Structure domains: Ribosomal protein S18
30S RIBOSOMAL PROTEIN S19 Chain: S
Molecule details ›
Chain: S
Length: 92 amino acids
Theoretical weight: 10.47 KDa
Source organism: Thermus thermophilus
Structure domains: 30s Ribosomal Protein S19; Chain A
30S RIBOSOMAL PROTEIN S20 Chain: T
Molecule details ›
Chain: T
Length: 105 amino acids
Theoretical weight: 11.6 KDa
Source organism: Thermus thermophilus
Structure domains: Ribosomal protein S20
30S RIBOSOMAL PROTEIN THX Chain: U
Molecule details ›
Chain: U
Length: 26 amino acids
Theoretical weight: 3.22 KDa
Source organism: Thermus thermophilus
16S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 1514 nucleotides
Theoretical weight: 491.51 KDa
Sequence domains: Bacterial small subunit ribosomal RNA

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID, ESRF BEAMLINE ID14-2, MPG/DESY, HAMBURG BEAMLINE BW6, EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P41212
Unit cell:
a: 406.3Å b: 406.3Å c: 173.1Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.203 0.245