1i92

X-ray diffraction
1.7Å resolution

STRUCTURAL BASIS OF THE NHERF PDZ1-CFTR INTERACTION

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Chain: A
Molecule details ›
Chain: A
Length: 91 amino acids
Theoretical weight: 9.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14745 (Residues: 11-99; Coverage: 25%)
Gene names: NHERF, NHERF1, SLC9A3R1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P3121
Unit cell:
a: 51.658Å b: 51.658Å c: 66.966Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.187 0.217
Expression system: Escherichia coli BL21(DE3)