1i7d

X-ray diffraction
2.05Å resolution

NONCOVALENT COMPLEX OF E.COLI DNA TOPOISOMERASE III WITH AN 8-BASE SINGLE-STRANDED DNA OLIGONUCLEOTIDE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA topoisomerase 3 Chain: A
Molecule details ›
Chain: A
Length: 659 amino acids
Theoretical weight: 74.14 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P14294 (Residues: 1-653; Coverage: 100%)
Gene names: JW1752, b1763, topB
Sequence domains:
Structure domains:
5'-D(*CP*GP*CP*AP*AP*CP*TP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.39 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: C2
Unit cell:
a: 122.011Å b: 60.831Å c: 125.358Å
α: 90° β: 90.73° γ: 90°
R-values:
R R work R free
0.233 0.233 0.26
Expression systems:
  • Escherichia coli BL21
  • Not provided