› 1i70

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF RNASE SA Y86F MUTANT

Released:
DOI: 10.2210/pdb1i70/pdb
PDB entry 1i70 coloured by chain, front view.
PDB entry 1i70 coloured by chain, side view.
PDB entry 1i70 coloured by chain, top view.
Source organism: Kitasatospora aureofaciens
Primary publication:
Tyrosine hydrogen bonds make a large contribution to protein stability.
Pace CN, Horn G, Hebert EJ, Bechert J, Shaw K, Urbanikova L, Scholtz JM, Sevcik J
J Mol Biol 312 393-404 (2001)
PMID: 11554795

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138777 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease Sa Chains: A, B
Molecule details ›
Chains: A, B
Length: 96 amino acids
Theoretical weight: 10.57 KDa
Source organism: Kitasatospora aureofaciens
Expression system: Escherichia coli
UniProt:
  • Canonical: P05798 (Residues: 1-96; Coverage: 100%)
Gene name: rnaSA
Sequence domains: ribonuclease
Structure domains: Microbial ribonucleases

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE D41A
Spacegroup: P212121
Unit cell:
a: 38.98Å b: 64.7Å c: 78.32Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.131 0.13 0.178
Expression system: Escherichia coli

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Citations

5 review citations

Protein structure, stability and solubility in water and other solvents.
Pace et al. (2004)
4 more