› 1i4a

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF PHOSPHORYLATION-MIMICKING MUTANT T6D OF ANNEXIN IV

Released:
DOI: 10.2210/pdb1i4a/pdb
PDB 1i4a coloured by chain and viewed from the front.
PDB 1i4a coloured by chain and viewed from the side.
PDB 1i4a coloured by chain and viewed from the top.
Source organism: Bos taurus
Primary publication:
Phosphorylation mutants elucidate the mechanism of annexin IV-mediated membrane aggregation.
Kaetzel MA, Mo YD, Mealy TR, Campos B, Bergsma-Schutter W, Brisson A, Dedman JR, Seaton BA
Biochemistry 40 4192-9 (2001)
PMID: 11300800

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Annexin A4 Chain: A
Molecule details ›
Chain: A
Length: 318 amino acids
Theoretical weight: 35.82 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13214 (Residues: 2-319; Coverage: 100%)
Gene names: ANX4, ANXA4
Sequence domains: Annexin
Structure domains: Annexin

Ligands and Environments

2 bound ligands:
Ligand CA 2 x CA
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: R3
Unit cell:
a: 119.06Å b: 119.06Å c: 82.16Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.23
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Annexins and Membrane Repair Dysfunctions in Muscular Dystrophies.
Croissant et al. (2021)
8 more

1 mention without citation

Conformational analysis of a genetically encoded FRET biosensor by SAXS.
Mertens et al. (2012)