PDBe 1hq1

X-ray diffraction
1.52Å resolution

STRUCTURAL AND ENERGETIC ANALYSIS OF RNA RECOGNITION BY A UNIVERSALLY CONSERVED PROTEIN FROM THE SIGNAL RECOGNITION PARTICLE

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Signal recognition particle protein Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 12.04 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AGD7 (Residues: 328-432; Coverage: 23%)
Gene names: JW5414, b2610, ffh
Sequence domains: Signal peptide binding domain
Structure domains: Signal recognition particle, SRP54 subunit, M-domain
4.5S RNA DOMAIN IV Chain: B
Molecule details ›
Chain: B
Length: 49 nucleotides
Theoretical weight: 15.93 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C2
Unit cell:
a: 136.555Å b: 78.337Å c: 32.872Å
α: 90° β: 96.24° γ: 90°
R-values:
R R work R free
0.157 0.151 0.199
Expression systems:
  • Escherichia coli
  • Not provided