› 1hh8

X-ray diffraction
1.8Å resolution

The active N-terminal region of p67phox: Structure at 1.8 Angstrom resolution and biochemical characterizations of the A128V mutant implicated in chronic granulomatous disease

Released:
DOI: 10.2210/pdb1hh8/pdb
PDB entry 1hh8 coloured by chain, front view.
PDB entry 1hh8 coloured by chain, side view.
PDB entry 1hh8 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The active N-terminal region of p67phox. Structure at 1.8 A resolution and biochemical characterizations of the A128V mutant implicated in chronic granulomatous disease.
Grizot S, Fieschi F, Dagher MC, Pebay-Peyroula E
J Biol Chem 276 21627-31 (2001)
PMID: 11262407

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148778 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neutrophil cytosol factor 2 Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 24.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19878 (Residues: 1-213; Coverage: 41%)
Gene names: NCF2, NOXA2, P67PHOX
Sequence domains: Tetratricopeptide repeat
Structure domains: Tetratricopeptide repeat domain

Ligands and Environments

1 bound ligand:
Ligand FLC 1 x FLC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P31
Unit cell:
a: 67.67Å b: 67.67Å c: 50.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.182 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

The NOX family of ROS-generating NADPH oxidases: physiology and pathophysiology.
Bedard et al. (2007)
12 more

11 mentions without citation

Activation and assembly of the NADPH oxidase: a structural perspective.
Groemping et al. (2005)
10 more