1hbx

X-ray diffraction
3.15Å resolution

Ternary Complex of SAP-1 and SRF with specific SRE DNA

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
Serum response factor Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 92 amino acids
Theoretical weight: 10.3 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11831 (Residues: 132-223; Coverage: 18%)
Gene name: SRF
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: Transcription factor, MADS-box
ETS domain-containing protein Elk-4 Chains: G, H
Molecule details ›
Chains: G, H
Length: 157 amino acids
Theoretical weight: 17.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28324 (Residues: 2-156; Coverage: 36%)
Gene names: ELK4, SAP1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*GP*AP*TP*GP*GP*CP*CP*TP*AP*AP*TP*TP*AP* GP*GP*AP*CP*TP*TP*CP*CP*GP*GP*TP*G)-3' Chains: C, F
Molecule details ›
Chains: C, F
Length: 26 nucleotides
Theoretical weight: 8.03 KDa
5'-D(*CP*AP*CP*AP*CP*CP*GP*GP*AP*AP*GP*TP*CP* CP*TP*AP*AP*TP*TP*AP*GP*GP*CP*CP*AP*T)-3' Chains: W, X
Molecule details ›
Chains: W, X
Length: 26 nucleotides
Theoretical weight: 7.94 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 142.68Å b: 144.39Å c: 75.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.252 0.252 0.285
Expression system: Escherichia coli BL21(DE3)