› 1h87

X-ray diffraction
1.72Å resolution

Gadolinium derivative of tetragonal Hen Egg-White Lysozyme at 1.7 A resolution

Released:
DOI: 10.2210/pdb1h87/pdb
PDB entry 1h87 coloured by chain, front view.
PDB entry 1h87 coloured by chain, side view.
PDB entry 1h87 coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Gd-HPDO3A, a complex to obtain high-phasing-power heavy-atom derivatives for SAD and MAD experiments: results with tetragonal hen egg-white lysozyme.
Girard E, Chantalat L, Vicat J, Kahn R
Acta Crystallogr D Biol Crystallogr 58 1-9 (2002)
PMID: 11752774

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

3 bound ligands:
Ligand DO3 2 x DO3
Ligand CL 4 x CL
Ligand GD 2 x GD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 77.25Å b: 77.25Å c: 38.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.18 0.18 0.21

Quick links

Citations

1 review citation

Artificial metalloenzymes constructed from hierarchically-assembled proteins.
Ueno et al. (2013)
15 other articles

9 mentions without citation

The R-factor gap in macromolecular crystallography: an untapped potential for insights on accurate structures.
Holton et al. (2014)
8 more