PDBe 1h2v

X-ray diffraction
2Å resolution

Structure of the human nuclear cap-binding-complex (CBC)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nuclear cap-binding protein subunit 1 Chain: C
Molecule details ›
Chain: C
Length: 771 amino acids
Theoretical weight: 89.67 KDa
Source organism: Homo sapiens
Expression system: Trichoplusia ni
UniProt:
  • Canonical: Q09161 (Residues: 20-790; Coverage: 98%)
Gene names: CBP80, NCBP, NCBP1
Sequence domains:
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Nuclear cap-binding protein subunit 2 Chain: Z
Molecule details ›
Chain: Z
Length: 156 amino acids
Theoretical weight: 18.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P52298 (Residues: 1-156; Coverage: 100%)
Gene names: CBP20, NCBP2, PIG55
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 264.136Å b: 59.605Å c: 75.43Å
α: 90° β: 99.52° γ: 90°
R-values:
R R work R free
0.212 0.212 0.247
Expression systems:
  • Trichoplusia ni
  • Escherichia coli BL21