1gvn

X-ray diffraction
1.95Å resolution

Crystal Structure of the Plasmid Maintenance System epsilon/zeta: Meachnism of toxin inactivation and toxin function

Released:

Function and Biology Details

Reaction catalysed:
ATP + UDP-N-acetyl-alpha-D-glucosamine = ADP + UDP-N-acetyl-alpha-D-glucosamine 3'-phosphate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Antitoxin epsilon Chains: A, C
Molecule details ›
Chains: A, C
Length: 90 amino acids
Theoretical weight: 10.73 KDa
Source organism: Streptococcus pyogenes
UniProt:
  • Canonical: Q57231 (Residues: 1-90; Coverage: 100%)
Sequence domains: Bacterial epsilon antitoxin
Structure domains: Helicase, Ruva Protein; domain 3
Toxin zeta Chains: B, D
Molecule details ›
Chains: B, D
Length: 287 amino acids
Theoretical weight: 32.44 KDa
Source organism: Streptococcus pyogenes
UniProt:
  • Canonical: Q54944 (Residues: 1-287; Coverage: 100%)
Sequence domains: Zeta toxin
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: P212121
Unit cell:
a: 59.236Å b: 79.847Å c: 191.439Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.2 0.237