PDBe 1gpp

X-ray diffraction
1.35Å resolution

Crystal structure of the S.cerevisiae Homing Endonuclease PI-SceI Domain I

Released:

Function and Biology Details

Reaction catalysed:
ATP + H(2)O + H(+)(In) = ADP + phosphate + H(+)(Out). 
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
V-type proton ATPase catalytic subunit A, 1st part Chain: A
Molecule details ›
Chain: A
Length: 237 amino acids
Theoretical weight: 27.03 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17255 (Residues: 283-466; Coverage: 17%)
Gene names: CLS8, D1286, TFP1, VMA1, YDL185W
Structure domains: Hedgehog/intein (Hint) domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: C2
Unit cell:
a: 102.856Å b: 47.595Å c: 60.297Å
α: 90° β: 121.41° γ: 90°
R-values:
R R work R free
0.153 0.15 0.189
Expression system: Escherichia coli