› 1got

X-ray diffraction
2Å resolution

HETEROTRIMERIC COMPLEX OF A GT-ALPHA/GI-ALPHA CHIMERA AND THE GT-BETA-GAMMA SUBUNITS

Released:
DOI: 10.2210/pdb1got/pdb
PDB entry 1got coloured by chain, front view.
PDB entry 1got coloured by chain, side view.
PDB entry 1got coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The 2.0 A crystal structure of a heterotrimeric G protein.
Lambright DG, Sondek J, Bohm A, Skiba NP, Hamm HE, Sigler PB
Nature 379 311-9 (1996)
PMID: 8552184

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136057 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Guanine nucleotide-binding protein G(t) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 350 amino acids
Theoretical weight: 40.69 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04695 (Residues: 1-350; Coverage: 100%)
Gene name: GNAT1
Sequence domains: G-protein alpha subunit
Structure domains:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 340 amino acids
Theoretical weight: 37.43 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P62871 (Residues: 1-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Guanine nucleotide-binding protein G(T) subunit gamma-T1 Chain: G
Molecule details ›
Chain: G
Length: 73 amino acids
Theoretical weight: 8.58 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P02698 (Residues: 2-66; Coverage: 88%)
Gene name: GNGT1
Sequence domains: GGL domain
Structure domains: Transducin (heterotrimeric G protein), gamma chain

Ligands and Environments

1 bound ligand:
Ligand GDP 1 x GDP
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 133.4Å b: 91.4Å c: 83.2Å
α: 90° β: 120.1° γ: 90°
R-values:
R R work R free
0.207 0.207 0.295
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

136 review citations

Integrins: bidirectional, allosteric signaling machines.
Hynes RO. (2002)
135 more

86 mentions without citation

G-protein signaling: back to the future.
McCudden et al. (2005)
85 more