PDBe 1go3

X-ray diffraction
1.75Å resolution

Structure of an archeal homolog of the eukaryotic RNA polymerase II RPB4/RPB7 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
DNA-directed RNA polymerase subunit E' Chains: E, M
Molecule details ›
Chains: E, M
Length: 187 amino acids
Theoretical weight: 21.25 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q57840 (Residues: 1-187; Coverage: 100%)
Gene names: MJ0397, rpoE1
Sequence domains:
Structure domains:
DNA-DIRECTED RNA POLYMERASE SUBUNIT F Chains: F, N
Molecule details ›
Chains: F, N
Length: 107 amino acids
Theoretical weight: 12.34 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q60351 (Residues: 9-115; Coverage: 93%)
Gene name: MJ0039
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43
Unit cell:
a: 92.391Å b: 92.391Å c: 91.109Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 0.238
Expression system: Escherichia coli BL21(DE3)