› 1glg

X-ray diffraction
2Å resolution

CRYSTALLOGRAPHIC ANALYSIS OF THE EPIMERIC AND ANOMERIC SPECIFICITY OF THE PERIPLASMIC TRANSPORT(SLASH)CHEMOTACTIC PROTEIN RECEPTOR FOR D-GLUCOSE AND D-GALACTOSE

Released:
DOI: 10.2210/pdb1glg/pdb
PDB entry 1glg coloured by chain, front view.
PDB entry 1glg coloured by chain, side view.
PDB entry 1glg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystallographic analysis of the epimeric and anomeric specificity of the periplasmic transport/chemosensory protein receptor for D-glucose and D-galactose.
Vyas MN, Vyas NK, Quiocho FA
Biochemistry 33 4762-8 (1994)
PMID: 8161535

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142447 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-galactose/methyl-galactoside binding periplasmic protein MglB Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 33.41 KDa
Source organism: Escherichia coli
Expression system: Not provided
UniProt:
  • Canonical: P0AEE5 (Residues: 24-332; Coverage: 100%)
Gene names: JW2137, b2150, mglB
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand GAL 1 x GAL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 65.98Å b: 37.09Å c: 61.9Å
α: 90° β: 105.83° γ: 90°
R-values:
R R work R free
0.167 not available not available
Expression system: Not provided

Quick links

Citations

3 review citations

Atomic structure and specificity of bacterial periplasmic receptors for active transport and chemotaxis: variation of common themes.
Quiocho et al. (1996)
2 more

1 mention without citation

The role of water molecules in stereoselectivity of glucose/galactose-binding protein.
Kim et al. (2016)