1gl2

X-ray diffraction
1.9Å resolution

Crystal structure of an endosomal SNARE core complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Vesicle-associated membrane protein 8 Chain: A
Molecule details ›
Chain: A
Length: 65 amino acids
Theoretical weight: 7.36 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WUF4 (Residues: 6-66; Coverage: 61%)
Gene name: Vamp8
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-7 Chain: B
Molecule details ›
Chain: B
Length: 65 amino acids
Theoretical weight: 7.33 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O70439 (Residues: 168-228; Coverage: 23%)
Gene names: Stx7, Syn7
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Vesicle transport through interaction with t-SNAREs homolog 1B Chain: C
Molecule details ›
Chain: C
Length: 65 amino acids
Theoretical weight: 7.55 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O88384 (Residues: 139-200; Coverage: 27%)
Gene names: Vti1b, Vti1l1
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Syntaxin-8 Chain: D
Molecule details ›
Chain: D
Length: 65 amino acids
Theoretical weight: 7.35 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Z2Q7 (Residues: 148-209; Coverage: 26%)
Gene name: Stx8
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SIEMENS M18X
Spacegroup: P21
Unit cell:
a: 50.7Å b: 41.1Å c: 51.5Å
α: 90° β: 109.4° γ: 90°
R-values:
R R work R free
0.176 0.176 0.224
Expression system: Escherichia coli