1gef

X-ray diffraction
2Å resolution

Crystal structure of the archaeal holliday junction resolvase HJC

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holliday junction resolvase Hjc Chains: A, B, D, E
Molecule details ›
Chains: A, B, D, E
Length: 123 amino acids
Theoretical weight: 13.79 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: E7FHX4 (Residues: 1-123; Coverage: 100%)
Gene names: PF1503, hjc
Sequence domains: Archaeal holliday junction resolvase (hjc)
Structure domains: Trna Endonuclease; Chain: A, domain 1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL24XU, SPRING-8 BEAMLINE BL40B2
Spacegroup: P21
Unit cell:
a: 36.245Å b: 119.056Å c: 63.146Å
α: 90° β: 90.66° γ: 90°
R-values:
R R work R free
0.224 0.224 0.26
Expression system: Escherichia coli BL21