1fxw

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF THE RECOMBINANT ALPHA1/ALPHA2 CATALYTIC HETERODIMER OF BOVINE BRAIN PLATELET-ACTIVATING FACTOR ACETYLHYDROLASE IB.

Released:

Function and Biology Details

Reaction catalysed:
1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H(2)O = 1-alkyl-sn-glycero-3-phosphocholine + acetate

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-549916 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Platelet-activating factor acetylhydrolase IB subunit alpha1 Chain: A
Molecule details ›
Chain: A
Length: 232 amino acids
Theoretical weight: 25.9 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q29460 (Residues: 1-232; Coverage: 100%)
Gene names: PAFAH1B3, PAFAHG
Sequence domains: GDSL-like Lipase/Acylhydrolase family
Structure domains: SGNH hydrolase
Platelet-activating factor acetylhydrolase IB subunit alpha2 Chain: F
Molecule details ›
Chain: F
Length: 229 amino acids
Theoretical weight: 25.6 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P68401 (Residues: 1-229; Coverage: 100%)
Gene names: PAFAH1B2P68402, PAFAHB
Sequence domains: GDSL-like Lipase/Acylhydrolase family
Structure domains: SGNH hydrolase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 44.519Å b: 80.848Å c: 116.207Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.218 0.275
Expression system: Escherichia coli