1fuu

X-ray diffraction
2.5Å resolution

YEAST INITIATION FACTOR 4A

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Crystal structure of yeast initiation factor 4A, a DEAD-box RNA helicase.
Proc. Natl. Acad. Sci. U.S.A. 97 13080-5 (2000)
PMID: 11087862

Function and Biology Details

Reaction catalysed:
ATP + H(2)O = ADP + phosphate
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATP-dependent RNA helicase eIF4A Chains: A, B
Molecule details ›
Chains: A, B
Length: 394 amino acids
Theoretical weight: 45.13 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P10081 (Residues: 2-395; Coverage: 100%)
Gene names: J0660, TIF1, TIF2, TIF41A, TIF41B, YJL138C, YKR059W
Sequence domains:
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P1
Unit cell:
a: 38.8Å b: 71.2Å c: 73.2Å
α: 94° β: 89.6° γ: 101°
R-values:
R R work R free
0.279 0.244 0.273
Expression system: Escherichia coli