PDBe 1fof

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF THE CLASS D BETA-LACTAMASE OXA-10

Released:
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure of the class D beta-lactamase OXA-10.
Nat. Struct. Biol. 7 918-25 (2000)
PMID: 11017203

Function and Biology Details

Reaction catalysed:
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase OXA-10 Chains: A, B
Molecule details ›
Chains: A, B
Length: 246 amino acids
Theoretical weight: 27.52 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: P14489 (Residues: 20-265; Coverage: 100%)
Gene names: bla, oxa10, pse2
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: P212121
Unit cell:
a: 48.4Å b: 96.2Å c: 125.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.177 0.208
Expression system: Escherichia coli