PDBe 1fo0

X-ray diffraction
2.5Å resolution

MURINE ALLOREACTIVE SCFV TCR-PEPTIDE-MHC CLASS I MOLECULE COMPLEX

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chain: H
Molecule details ›
Chain: H
Length: 276 amino acids
Theoretical weight: 31.91 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01901 (Residues: 22-296; Coverage: 79%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chain: L
Molecule details ›
Chain: L
Length: 99 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
BTB/POZ domain-containing protein KCTD20 Chain: P
Molecule details ›
Chain: P
Length: 8 amino acids
Theoretical weight: 983 Da
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q8CDD8 (Residues: 207-214; Coverage: 2%)
Gene name: Kctd20
TRADV16D Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 12.95 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: Q5R1F1 (Residues: 23-116; Coverage: 98%)
Gene names: TRADV16D, TRAV16
Structure domains: Immunoglobulins
T-cell receptor beta chain V region E1 Chain: B
Molecule details ›
Chain: B
Length: 112 amino acids
Theoretical weight: 12.97 KDa
Source organism: Mus musculus
Expression system: Mus musculus
UniProt:
  • Canonical: P04214 (Residues: 22-133; Coverage: 95%)
Structure domains: Immunoglobulins

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P21212
Unit cell:
a: 76.58Å b: 120.42Å c: 102.85Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.225 0.276
Expression systems:
  • Escherichia coli
  • Not provided
  • Mus musculus