› 1fhx

X-ray diffraction
2.5Å resolution

Structure of the pleckstrin homology domain from GRP1 in complex with inositol 1,3,4,5-tetrakisphosphate

Released:
DOI: 10.2210/pdb1fhx/pdb
PDB entry 1fhx coloured by chain, front view.
PDB entry 1fhx coloured by chain, side view.
PDB entry 1fhx coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural basis for discrimination of 3-phosphoinositides by pleckstrin homology domains.
Ferguson KM, Kavran JM, Sankaran VG, Fournier E, Isakoff SJ, Skolnik EY, Lemmon MA
Mol Cell 6 373-84 (2000)
PMID: 10983984

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-126701 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytohesin-3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 129 amino acids
Theoretical weight: 15.36 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: O08967 (Residues: 264-391; Coverage: 32%)
Gene names: Cyth3, Grp1, Pscd3
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

2 bound ligands:
Ligand 4IP 2 x 4IP
Ligand SO4 2 x SO4
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P212121
Unit cell:
a: 56.09Å b: 64.54Å c: 69.73Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.286
Expression system: Escherichia coli

Quick links

Citations

43 review citations

The activation of Akt/PKB signaling pathway and cell survival.
Song et al. (2005)
42 more

6 mentions without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)
5 more