› 1ffw

X-ray diffraction
2.7Å resolution

CHEY-BINDING DOMAIN OF CHEA IN COMPLEX WITH CHEY WITH A BOUND IMIDO DIPHOSPHATE

Released:
DOI: 10.2210/pdb1ffw/pdb
PDB entry 1ffw coloured by chain, front view.
PDB entry 1ffw coloured by chain, side view.
PDB entry 1ffw coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Further insights into the mechanism of function of the response regulator CheY from crystallographic studies of the CheY--CheA(124--257) complex.
Gouet P, Chinardet N, Welch M, Guillet V, Cabantous S, Birck C, Mourey L, Samama JP
Acta Crystallogr D Biol Crystallogr 57 44-51 (2001)
PMID: 11134926

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139568 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chemotaxis protein CheY Chains: A, C
Molecule details ›
Chains: A, C
Length: 128 amino acids
Theoretical weight: 13.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AE67 (Residues: 2-129; Coverage: 99%)
Gene names: JW1871, b1882, cheY
Sequence domains: Response regulator receiver domain
Structure domains: Rossmann fold
Chemotaxis protein CheA Chains: B, D
Molecule details ›
Chains: B, D
Length: 134 amino acids
Theoretical weight: 14.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P07363 (Residues: 124-257; Coverage: 21%)
Gene names: JW1877, b1888, cheA
Sequence domains: CheY binding
Structure domains: CheY-binding domain of CheA

Ligands and Environments

2 bound ligands:
Ligand MN 2 x MN
Ligand PON 1 x PON
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P212121
Unit cell:
a: 159.3Å b: 53.4Å c: 77.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.273
Expression system: Escherichia coli

Quick links

Citations

14 citation in other articles

Structure of the ternary complex formed by a chemotaxis receptor signaling domain, the CheA histidine kinase, and the coupling protein CheW as determined by pulsed dipolar ESR spectroscopy.
Bhatnagar et al. (2010)
13 more

17 mentions without citation

Protein-protein docking benchmark version 4.0.
Hwang et al. (2010)
16 more