› 1fd9

X-ray diffraction
2.41Å resolution

CRYSTAL STRUCTURE OF THE MACROPHAGE INFECTIVITY POTENTIATOR PROTEIN (MIP) A MAJOR VIRULENCE FACTOR FROM LEGIONELLA PNEUMOPHILA

Released:
DOI: 10.2210/pdb1fd9/pdb
PDB entry 1fd9 coloured by chain, front view.
PDB entry 1fd9 coloured by chain, side view.
PDB entry 1fd9 coloured by chain, top view.
Source organism: Legionella pneumophila
Primary publication:
Crystal structure of Mip, a prolylisomerase from Legionella pneumophila.
Riboldi-Tunnicliffe A, König B, Jessen S, Weiss MS, Rahfeld J, Hacker J, Fischer G, Hilgenfeld R
Nat Struct Biol 8 779-83 (2001)
PMID: 11524681

Function and Biology Details

Reaction catalysed: 
Peptidylproline (omega=180) = peptidylproline (omega=0)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178704 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Outer membrane protein MIP Chain: A
Molecule details ›
Chain: A
Length: 213 amino acids
Theoretical weight: 22.87 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5ZXE0 (Residues: 21-233; Coverage: 100%)
Gene names: lpg0791, mip
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ELETTRA BEAMLINE 5.2R
Spacegroup: P43212
Unit cell:
a: 80.75Å b: 80.75Å c: 103.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.28
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Molecular pathogenesis of infections caused by Legionella pneumophila.
Newton et al. (2010)
6 more

17 mentions without citation

Microbial peptidyl-prolyl cis/trans isomerases (PPIases): virulence factors and potential alternative drug targets.
Ünal et al. (2014)
16 more