› 1ehb

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF RECOMBINANT TRYPSIN-SOLUBILIZED FRAGMENT OF CYTOCHROME B5

Released:
DOI: 10.2210/pdb1ehb/pdb
PDB 1ehb coloured by chain and viewed from the front.
PDB 1ehb coloured by chain and viewed from the side.
PDB 1ehb coloured by chain and viewed from the top.
Source organism: Bos taurus
Primary publication:
Crystal structure of recombinant trypsin-solubilized fragment of cytochrome b(5) and the structural comparison with Val61His mutant.
Wu J, Gan JH, Xia ZX, Wang YH, Wang WH, Xue LL, Xie Y, Huang ZX
Proteins 40 249-57 (2000)
PMID: 10842340

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b5 Chain: A
Molecule details ›
Chain: A
Length: 82 amino acids
Theoretical weight: 9.48 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P00171 (Residues: 8-89; Coverage: 61%)
Gene names: CYB5, CYB5A
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 70.7Å b: 40.44Å c: 39.28Å
α: 90° β: 111.76° γ: 90°
R-values:
R R work R free
0.198 0.198 0.248
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

1H NMR studies of the effect of mutation at Valine45 on heme microenvironment of cytochrome b5.
Cao et al. (2003)
3 more

3 mentions without citation

Modeling the backbone dynamics of reduced and oxidized solvated rat microsomal cytochrome b5.
Giachetti et al. (2004)
2 more