1dux

X-ray diffraction
2.1Å resolution

ELK-1/DNA STRUCTURE REVEALS HOW RESIDUES DISTAL FROM DNA-BINDING SURFACE AFFECT DNA-RECOGNITION

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ETS domain-containing protein Elk-1 Chains: C, F
Molecule details ›
Chains: C, F
Length: 94 amino acids
Theoretical weight: 11.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19419 (Residues: 1-94; Coverage: 22%)
Gene name: ELK1
Sequence domains: Ets-domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*TP*GP*AP*CP*CP*GP*GP*AP*AP*GP*TP*GP*T)-3') Chains: A, D
Molecule details ›
Chains: A, D
Length: 13 nucleotides
Theoretical weight: 4.03 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*AP*CP*AP*CP*TP*TP*CP*CP*GP*GP*TP*CP*A)-3') Chains: B, E
Molecule details ›
Chains: B, E
Length: 13 nucleotides
Theoretical weight: 3.91 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 33.245Å b: 140.523Å c: 38.62Å
α: 90° β: 115.52° γ: 90°
R-values:
R R work R free
0.202 0.202 0.236
Expression systems:
  • Escherichia coli
  • Not provided