› 1dtl

X-ray diffraction
2.15Å resolution

CRYSTAL STRUCTURE OF CALCIUM-SATURATED (3CA2+) CARDIAC TROPONIN C COMPLEXED WITH THE CALCIUM SENSITIZER BEPRIDIL AT 2.15 A RESOLUTION

Released:
DOI: 10.2210/pdb1dtl/pdb
PDB entry 1dtl coloured by chain, front view.
PDB entry 1dtl coloured by chain, side view.
PDB entry 1dtl coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Bepridil opens the regulatory N-terminal lobe of cardiac troponin C.
Li Y, Love ML, Putkey JA, Cohen C
Proc Natl Acad Sci U S A 97 5140-5 (2000)
PMID: 10792039

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140775 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Troponin C, slow skeletal and cardiac muscles Chain: A
Molecule details ›
Chain: A
Length: 161 amino acids
Theoretical weight: 18.4 KDa
Source organism: Gallus gallus
Expression system: Escherichia coli
UniProt:
  • Canonical: P09860 (Residues: 1-161; Coverage: 100%)
Gene name: TNNC1
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand BEP 3 x BEP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P212121
Unit cell:
a: 36.27Å b: 62.04Å c: 62.04Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.276
Expression system: Escherichia coli

Quick links

Citations

9 review citations

Structural basis for diversity of the EF-hand calcium-binding proteins.
Grabarek Z. (2006)
8 more

10 mentions without citation

Structure and function of cardiac troponin C (TNNC1): Implications for heart failure, cardiomyopathies, and troponin modulating drugs.
Li et al. (2015)
9 more