PDBe 1dig

X-ray diffraction
2.2Å resolution

HUMAN METHYLENETETRAHYDROFOLATE DEHYDROGENASE / CYCLOHYDROLASE COMPLEXED WITH NADP AND INHIBITOR LY374571

Released:

Function and Biology Details

Reactions catalysed:
5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate
5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
C-1-tetrahydrofolate synthase, cytoplasmic, N-terminally processed Chains: A, B
Molecule details ›
Chains: A, B
Length: 306 amino acids
Theoretical weight: 33.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P11586 (Residues: 1-306; Coverage: 33%)
Gene names: MTHFC, MTHFD, MTHFD1
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 2 x NAP
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P212121
Unit cell:
a: 67.889Å b: 136.419Å c: 61.579Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.267
Expression system: Escherichia coli