1df1

X-ray diffraction
2.35Å resolution

MURINE INOSOXY DIMER WITH ISOTHIOUREA BOUND IN THE ACTIVE SITE

Released:

Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nitric oxide synthase, inducible Chains: A, B
Molecule details ›
Chains: A, B
Length: 423 amino acids
Theoretical weight: 49 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29477 (Residues: 77-499; Coverage: 37%)
Gene names: Inosl, Nos2
Sequence domains: Nitric oxide synthase, oxygenase domain
Structure domains:

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P6122
Unit cell:
a: 214.464Å b: 214.464Å c: 112.752Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.223 0.223 0.298
Expression system: Escherichia coli