1czz

X-ray diffraction
2.7Å resolution

STRUCTURE OF TNF RECEPTOR ASSOCIATED FACTOR 2 IN COMPLEX WITH A 17-RESIDUE CD40 PEPTIDE

Released:
Source organism: Homo sapiens

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only pentamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TNF receptor-associated factor 2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 187 amino acids
Theoretical weight: 21.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12933 (Residues: 315-501; Coverage: 37%)
Gene names: TRAF2, TRAP3
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
Tumor necrosis factor receptor superfamily member 5 Chains: D, E
Molecule details ›
Chains: D, E
Length: 10 amino acids
Theoretical weight: 1.01 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P25942 (Residues: 250-258; Coverage: 4%)
Gene names: CD40, TNFRSF5

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 59.4Å b: 81.1Å c: 77.2Å
α: 90° β: 96.8° γ: 90°
R-values:
R R work R free
0.221 0.221 0.267
Expression systems:
  • Escherichia coli
  • Not provided