1cmi

X-ray diffraction
2.5Å resolution

STRUCTURE OF THE HUMAN PIN/LC8 DIMER WITH A BOUND PEPTIDE

Released:
Source organisms:
Primary publication:
Structure of the PIN/LC8 dimer with a bound peptide.
Nat. Struct. Biol. 6 735-40 (1999)
PMID: 10426949

Function and Biology Details

Reaction catalysed:
(1a) 2 L-arginine + 2 NADPH + 2 O(2) = 2 N(omega)-hydroxy-L-arginine + 2 NADP(+) + 2 H(2)O

Structure analysis Details

Assemblies composition:
hetero hexamer
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Dynein light chain 1, cytoplasmic Chains: A, B
Molecule details ›
Chains: A, B
Length: 85 amino acids
Theoretical weight: 9.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63167 (Residues: 5-89; Coverage: 96%)
Gene names: DLC1, DNCL1, DNCLC1, DYNLL1, HDLC1
Sequence domains: Dynein light chain type 1
Structure domains: Protein Inhibitor Of Neuronal Nitric Oxide Synthase;
Nitric oxide synthase, brain Chains: C, D
Molecule details ›
Chains: C, D
Length: 13 amino acids
Theoretical weight: 1.49 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
UniProt:
  • Canonical: P29476 (Residues: 225-237; Coverage: 1%)
Gene names: Bnos, Nos1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: I4122
Unit cell:
a: 67.2Å b: 67.2Å c: 217Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.283
Expression systems:
  • Escherichia coli BL21
  • Not provided