1cjr

X-ray diffraction
2.3Å resolution

X-RAY CRYSTALLOGRAPHIC STUDIES OF DENATURATION IN RIBONUCLEASE S

Released:

Function and Biology Details

Reaction catalysed:
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-158391 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 15 amino acids
Theoretical weight: 1.75 KDa
Source organism: synthetic construct
UniProt:
  • Canonical: P61823 (Residues: 27-41; Coverage: 12%)
Gene names: RNASE1, RNS1
Ribonuclease pancreatic Chain: B
Molecule details ›
Chain: B
Length: 101 amino acids
Theoretical weight: 11.29 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 50-150; Coverage: 82%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P3121
Unit cell:
a: 44.65Å b: 44.65Å c: 98.1Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.245 0.245 0.27