PDBe 1cbo

X-ray diffraction
1.8Å resolution

CHOLESTEROL OXIDASE FROM STREPTOMYCES HIS447ASN MUTANT

Released:

Function and Biology Details

Reactions catalysed:
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholesterol oxidase Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 54.95 KDa
Source organism: Streptomyces sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: P12676 (Residues: 43-546; Coverage: 100%)
Gene name: choA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 51.58Å b: 74.15Å c: 63.74Å
α: 90° β: 105.08° γ: 90°
R-values:
R R work R free
0.159 0.159 0.194
Expression system: Escherichia coli